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Enzymes for Glycobiology - Overview
IBEX produces a family of high purity recombinant glycosaminoglycan (GAG) lyases; Heparinase I, Heparinase II, Heparinase III, Chondroitinase AC and Chondroitinase B.
Although these enzymes are available from the native strain of Flavobacterium heparinum (also known as Pedobacter heparinus), enzymes which are purified from the native strain will almost certainy contain cross-contaminants of other flavo enzymes.
IBEX has developed a proprietary expression system in Flavobacterium heparinum and can produce these enzymes at the highest purity.
Using pure enzymes will improve the quality of your results.
| Enzyme |
Substrate |
Heparinase I (EC 4.2.2.7) (ratio approx. 3:1). Eliminative cleavage of polysaccharides containing 1,4-linked D-glucuronate or L-iduronate residues and 1,4-a-linked 2-sulfoamino-2-deoxy-6-sulfo-D-glucose residues to give oligosaccharides with terminal 4-deoxy-a-D-gluc-4-enuronosyl groups at their non-reducing ends Heparinase I cleaves heparin, and to a lesser extent, heparan sulfate. (MSDS Sheet).
Data Sheet
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Heparinase II (UEC 4.2.2.19) Heparinase II cleaves heparin and heparan sulfate.
Data Sheet
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Heparinase III (EC 4.2.2.8) Heparinase III cleaves heparan sulfate exclusively, and does not cleave unfractionated heparin or low molecular weight heparins.
Data Sheet |
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Chondroitinase AC(EC 4.2.2.5) Eliminative degradation of polysaccharides containing 1,4-b-D-hexosaminyl and 1,3-b-D-glucuronosyl linkages to disaccharides containing 4-deoxy-b-D-gluc-4-enuronosyl groups. Chondroitinase AC cleaves chondroitin sulfates A and C.
Data Sheet |
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Chondroitinase B (unassigned EC) Chondroitinase B cleaves dermatan sulfate.
Data Sheet
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Every unit is composed of uronic acid (D-GlcA or L-IdoA) and amino sugar (D-GalNAc or D-GlcNH2). R, H or SO3- ; R', H, COCH3 or SO3-. DS also contains some CS disaccharide units. HS also contains some HP disaccharide units and vice versa.
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